ReadCrystal’s biophysical detection platform offers comprehensive methods for the detection of protein-protein, protein-small molecule, protein-nucleic acid, and other molecular interactions. This platform is used to validate the affinity and stability between candidate small molecules and target proteins, optimize drug safety and efficacy, and promote lead compound confirmation.
Surface Plasmon Resonance(SPR)
Micro-Scale Thermophoresis(MST)
Isothermal Titration Calorimetry(ITC)
Bio-Layer Interferometry (BLI)
Nano Differential Scanning Fluorimetry(Nano-DSF)
Thermal Shift Assay(TSA)
Surface Plasmon Resonance(SPR)
- Unlabeled samples
- Binding constant Kon
- High throughput
- Dissociation constant Koff
- Measuring affinity (Kd)
Micro-Scale Thermophoresis(MST)
- No need for sample fixation
- Extremely low sample consumption
- Measuring affinity (Kd)
- Not limited by ligand molecular weight
Isothermal Titration Calorimetry(ITC)
- No need for sample labeling or fixation
- Measuring affinity (Kd)
- Reaction enthalpy and entropy changes
- Binding site numbers
Bio-Layer Interferometry (BLI)
- Unlabeled samples
- High throughput
- Measuring affinity (Kd)
- Binding constant Kon
- Dissociation constant Koff
Nano Differential Scanning Fluorimetry(Nano-DSF)
- Not limited by sample buffer constraints
- High throughput
- Melting temperature (Tm)
- The intrinsic thermal stability of the target protein
- Ligand-target protein interactions
Thermal Shift Assay(TSA)
- No need for sample fixation
- High throughput
- Melting temperature (Tm)
- The intrinsic thermal stability of the target protein
- Ligand-target protein interactions
