ASMS

ASMS

ASMS

Affinity Selection Mass Spectrometry (ASMS) is an innovative screening technology that combines affinity selection with high-resolution mass spectrometry to identify and characterize small molecule interactions with target proteins.

Key Features

High Throughput

Compared with traditional drug screening methods, ASMS can process hundreds to thousands of compounds in a single run, enabling rapid screening of large-scale compound libraries.

Wide range of Applications

It can be applied to the screening of various types of targets, including membrane proteins, nucleic acids and protein complexes. There is no need to introduce tags, allowing for screening in a native state.

No Preference of Mechanism

It can indiscriminately detect various types of target-ligand interactions without relying on radioactive, fluorescent labeling, or enzymatic activity methods.

Technical Workflow

01

Target Acquisition & Library Selection

Determine the biological target to be screened, such as a protein, RNA, or complex, and ensure the purity and functional integrity of the target.
Select an appropriate compound library for screening based on the target's characteristics and the client's requirements.

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02

Incubation & Isolation

Incubate the target with a mixture of small molecules from the compound library to form complexes, which are then separated using techniques such as Ultrafiltration, Magnetic beads, or size-exclusion chromatography (SEC).

03

Mass Spectrometry Analysis

After denaturing the complexes to obtain the bound small molecules, detection is performed using a high-resolution mass spectrometer.

04

Interpretation of Results

Analyze the mass spectrometry data to identify the small molecules that bind to the target. Follow-up options include hit validation, determination of compound affinity (KD), and studies on the binding mechanism.