Protein Production

Protein is the core of Biology. Self-built recombinant protein product gallery with target proteins as the core, focusing on kinases, membrane proteins, ubiquitination enzymes, GTases, hydrolases, transcription factors, etc., required for innovative drug research and development, and constantly expand our product accumulation. Moreover, customized plan can be provided.

> %

Protein Purity >90%

More Than 300 Proteins

Custom

Support For Customisation

Type of protein

Technical Workflow

Protein Gallery

Gene Synthesis & Plasmid Construction

Various purification tags and plasmid types meets requirements for its intended crystallization,
affinity determination, functional studies and more.

Protein Expression

E.coli expression system: BL21, Rossetta, T7, etc.
Insect cells expression system: Sf9, Sf21, High Five, etc.
Mammalian cells expression system: HEK293, CHO, etc.
Other systems: Yeast, Cell-free
20+ vectors suitable for different expression systems
20+ purification tags for next purification step

Protein Purification

Integrated chromatographic purification techniques: affinity, ion exchange, hydrophobic interaction,
and size-exclusion chromatography
ÄKTA purification equipment
Guaranteed protein purity over 90%

Plasmid Construction

Carrier Type

Purification Label

pET28a	pETDuet-1	pcDNA3.1	pColdTF
pET22b	pACYCDuet-1	pFastbac	pVB322
pGEX-6p-1	pCDFDuet-1	pFastbacDuet	……

His-GST-PreScission	His-strep tag II -PreScission	His-protein A	GP67-His-sumostar-TEV
His-GST-TEV	His-CL7-PreScission	thrombin-Fc	FLAG-avi
His-TEV	His-MBP-pression	His-PreScission-strep-TEV	His-PreScission-strep-TEV-FLAG
His-PreScission	His-sumo-PreScission	His-GFP-PreScission	mcherry-TEV
His-Trx-PreScission	His-TEV-EGFP	GP67-His-PreScission	.....
His-Dsba-PreScission	His-TEV-EGFP-PreScission	GP67-His-sumostar-PreScission

Expression Systems

Prokaryotic Expression System

Insect Expression System

Mammalian Cell Expression System

Yeast Expression System

Prokaryotic Expression System

Escherichia coli strains such as BL21 and Rosetta are commonly used. Other potential bacterial hosts include Bacillus subtilis and Streptomyces.

We possess a diverse range of expression vectors that are compatible with optimal strains. We continuously optimize expression conditions and refolding processes to achieve the highest yield parameter combinations.

The system is suitable for：Non-glycosylated recombinant proteins、Recombinant proteins with simple modifications、Isotopically labeled proteins.

Advantages：

- Mature technology, widely applied, low cost

- Rapid reproduction, high expression levels, shortened experimental time

- Strong resistance to contamination

- Relatively simple separation and purification of expression products, good stability

- Clear genetic background

Insect Expression System

Also known as the Baculovirus Expression Vector System (BEVS), it is the expression system closest to mammalian cells.

Our multi-system and multi-vector expression strategy can overcome various challenges in traditional expression systems, maximizing expression efficiency and quality.

The system is suitable for：Viral vaccines、Signal molecules、Cytokines、Kinases, etc.

Advantages：

- Large gene capacity and high efficiency in expressing foreign genes

- Glycosylation and extensive post-translational modifications

- Easy screening of recombinant viruses

- Expression of soluble proteins

- Endotoxin-free

Mammalian Cell Expression System

The mammalian cell expression system provides recombinant human proteins with post-translational modifications, protein folding, and aggregation that are closest to their natural state. It ensures the spatial structure and modifications necessary for active proteins.

We possess unique mammalian cell expression vectors and a combination of various transfection methods. Customized solutions are designed based on the target protein, optimizing expression conditions to significantly increase yield and shorten experimental cycles.

The system is suitable for：Vaccines、Antibodies, etc.

Advantages：

- High expression levels

- Accurate protein folding and post-translational modifications

- Capable of expressing secretory proteins

- Suspension culture characteristics allow for large-scale production

- Endotoxin-free

Yeast Expression System

The yeast expression system is divided into the Saccharomyces cerevisiae (brewer's yeast) expression system and the methylotrophic yeast expression system. The latter, such as Pichia pastoris, is currently the most widely used yeast expression system.

By utilizing our proprietary vector-host combinations, we can significantly enhance the quality and quantity of recombinant protein expression and shorten the experimental cycle.

The system is suitable for：Secretory proteins、Recombinant proteins with simple modifications、Isotopically labeled proteins, etc.

Advantages：

- Short cycle, low cost, high efficiency

- Capable of extensive protein processing and post-translational modifications

- Suitable for expressing secretory proteins

- Endotoxin-free

Techniques Purification

Understand the properties of the target protein and major impurities in the sample to be purified. Based on the protein's origin, characteristics (molecular size, isoelectric point), and stability (tolerance to temperature, extreme pH, proteases, oxygen, and metal ions), design the optimal protein purification strategy.

High-Pressure Low-Temperature Homogenization

After cell harvesting, large-scale cell disruption is achieved through liquid shear forces to obtain cellular material for subsequent purification.

Gel Filtration Chromatography

Also known as size-exclusion chromatography or molecular sieving. This technique separates components based on differences in their relative molecular masses. The operating conditions are relatively mild and do not require organic solvents.

Affinity Chromatography

Protein separation and purification are achieved through specific interactions between biomolecules. Ligands on the affinity adsorbent selectively bind to substances that can interact with them. This method is characterized by high selectivity, high purity, and rapidity. It is commonly used as the first step in the purification of recombinant proteins to remove most impurities.

Ion Exchange Chromatography

Different proteins have different isoelectric points and carry different charges under varying pH buffer conditions. Ion exchange columns can be selected based on the protein's charge properties. The process is reversible and highly controllable, often used in combination with other methods in the final purification steps.

Hydrophobic Interaction Chromatography

This is a chromatography method that purifies biomolecules based on their hydrophobic properties. Under high ionic strength conditions, the hydrophobic interactions between proteins and the chromatography medium are enhanced, allowing adsorption. When the ionic strength of the mobile phase is reduced, the hydrophobic interactions between the protein molecules and the medium weaken, and the target protein can then be eluted and collected.