X-Ray Crystallography

X-Ray Crystallography

X-Ray Crystallography

X-Ray Crystallography is a powerful method that uses the interaction of X-rays with crystallized proteins to reveal their high-resolution structures, essential for understanding their functions.

Suitable for

Small to medium-sized proteins forming good crystals

Some membrane proteins with proper crystallization

Mw <100kda

Stable proteins without conformational changes in crystals (e.g., enzymes, globular proteins)

Gallery Structures

Gallery entry

MTD3-MTD14，1.5Å

300+ predefined protein structures in RC’s database ensure rapid, reliable and accurate result possibly within 1 month

Literature Structures

PRMT5，2.8Å

One-stop gene-to-structure service is ready to provide in timeline of 1-3 months.

Novel Structures

PrfaH，2.0Å

Unmatched experienced scientists are here to design and tailor your highly novel target.

Technical Workflow

01

Crystallization-grade Protein Production

Gene contrust design
Protein expression
Protein purification

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02

Initial 96-well Crystallization Screening

High-throughput screening
2000+ conditions tested in parallel
Mosquito robot ensure scalability and precision

03

Optimization of Crystallization Hit

The crystal parameters were fine-tuned, the crystal quality and solid form of the crystals were adjusted, so as to
obtain the optimal results for the subsequent structural study
Ultraviolet CrysCam is used to identify whether the crystals are salt or protein

04

Synchrotron Radiation Data Collection

Collaborations with domestic and global synchrotron beamline: Shanghai Synchrotron Radiation Facility,
Spring 8, Diamond, etc. for privileged access for data collection for sufficient machine hours.

05

Structure determination, modeling and refinement

Stringent quality control process for all structures delivered (PDB standard)
Software(Phenix, CCP4)
MIR/MAD/SAD and Molecular Replacement

Case Studies

Imine Reductase(IRED)

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SHP2-SHP099

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