The Embryonic Ectoderm Development (EED) protein was initially discovered to regulate the activity of PRC2 by recognizing histone modifications to maintain gene silencing. As one of the assembly scaffolds of the PRC2 complex, it is associated with various cancers, including breast cancer, colorectal cancer, prostate cancer, B-cell lymphoma (DLBCL), and follicular lymphoma (FL). Therefore, it has become an important target for the development of epigenetic drugs.

ReadCrystal has completed the structural analysis of the EED protein in complex with small molecules. It has accumulated rich experience in protein expression and purification as well as in the structural analysis of inhibitor co-crystals for this target. Based on these experimental conditions and stock proteins, we have shortened the project delivery time to 1–2 months and ensured the successful delivery of the project.

EED Protein

The Embryonic Ectoderm Development (EED), initially identified as a key member of the Polycomb Group (PcG) in studies of Drosophila embryonic development, was later found to be one of the core subunits of the Polycomb Repressive Complex 2 (PRC2) (Figure 1).

The Polycomb Group (PcG) is a class of transcriptional repressors that are highly conserved in evolution and are related to growth and development. Their mechanism of action mainly involves modifying histones or altering chromatin structure to cause the silencing of expression of homeotic genes or developmentally regulated genes.

The PcG has multiple family members, among which the main ones that exert transcriptional repression functions are PRC1 and PRC2. These two complexes are composed of different catalytic and regulatory subunits. The PRC2 protein complex has methyltransferase activity and can catalyze the di- and tri-methylation modification reactions of histone H3K27.

Figure 1 Schematic diagram of the PRC2 complex structure

Our Capability

EED Protein Experimental Results

Protein Purification Result(Period: In Stock/2 weeks)

Figure 2 EED Protein Purification Result(without small molecule)

Crystallization and Structural Analysis(Period: 1-2 weeks)